Coding

Part:BBa_K5089008:Design

Designed by: Camille Prefontaine   Group: iGEM24_UManitoba   (2024-09-29)


ABO2449(S146R)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 735
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 754
    Illegal NgoMIV site found at 874
    Illegal NgoMIV site found at 900
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The sequence for this part is codon-optimized for expression in E. coli.


Source

Alcanivorax borkumensis, gene number 2449

References

1. Chen, A., Xu, T., Ge, Y., Wang, L., Tang, W., & Li, S. 2019. Hydrogen-bond-based protein engineering for the acidic adaptation of Bacillus acidopullulyticus pullulanase. Enzyme and Microbial Technology, 124, 79–83. https://doi.org/10.1016/j.enzmictec.2019.01.010